Characterization of mesenchymal stromal cells physical properties using acoustic radiation force

Microfluidics-based technologies are emerging solutions towards cell separation process. They rely on various physical principles, such as dielectrophoretic force, hydrodynamic and acoustic force. In order to take advantage of these approaches for sorting, it is crucial characterize biophysical properties, i.e. size, density, compressibility or contrast factor in the case acoustophoresis. Various techniques exist measure those features, including acoustofluidics methods. However, previously described do not allow determination all parameters a given cell. For this study, 330 μ m deep cavity has been designed, together with an optical technique, density cells finally determine their compressibility. A defocusing technique used assess velocity sedimentation focusing individual which can lead properties using analytic expression Acoustic Radiation Force. This method investigate evolution Mesenchymal Stromal Cells (MSCs), isolated from two different tissues donors, one passage other. Our results highlighted important heterogeneity among same donor but also inter-donor. Nevertheless, passage, MSCs converge homogenize at fourth passage. On other hand, remains homogenous between donors. Using was possible successfully mesenchymal stromal single setup based levitation. The confirm necessity use properties. It demonstrates large donor/patient-derived cells, cultured whose during successive cultures. As consequence, processes will be more complex patient/donor derived than cells.